Partition of tRNAGly isoacceptors between protein and cell-wall peptidoglycan synthesis in Staphylococcus aureus
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چکیده
منابع مشابه
Ristocetins, inhibitors of cell wall synthesis in Staphylococcus aureus.
Inhibition of bacterial cell wall synthesis is an important mechanism of action of antibacterial agents. Bacterial cell walls are chemically and morphologically distinct from any structures found in animal cells. Inhibition of synthesis of these structures results, therefore, in selective toxicity. The antibacterial action of penicillins (1, 2), n-cycloserine (3, 4), bacitracin (5), and novobio...
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The biosynthesis of pentaglycine, the cross-linking peptide in the cell wall peptidoglycan of Sfaphylococcus aureus, requires glycyl-tRNA as a donor of glycine. The pentaglycine is introduced by sequential addition of glycine from glycyltRNA to the e-amino group of the L-lysine residue of the pentapeptide in the lipid-linked precursor. The direction of elongation of the pentaglycine chain is fr...
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Staphylococcus simulans bv. staphylolyticus secretes lysostaphin, a bacteriocin that cleaves pentaglycine cross bridges in the cell wall of Staphylococcus aureus. The C-terminal cell wall-targeting domain (CWT) of lysostaphin is required for selective binding of this bacteriocin to S. aureus cells; however, the molecular target for this was unknown. We used purified green fluorescent protein fu...
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Cell-free Staphylococcus aureus extracts have been prepared which actively incorporate amino acids into protein. The requirements for amino acid incorporation of this preparation were strongly suggestive of de novo protein synthesis, since it showed an absolute requirement for ribosomes, 105,000 x g supernatant fluid, energy source, and magnesium ion. The stability of these extracts was greatly...
متن کاملFunctional interrelationships between cell membrane and cell wall in antimicrobial peptide-mediated killing of Staphylococcus aureus.
Perturbation of the Staphylococcus aureus cytoplasmic membrane (CM) is felt to play a key role in the microbicidal mechanism of many antimicrobial peptides (APs). However, it is not established whether membrane permeabilization (MP) alone is sufficient to kill susceptible staphylococci or if the cell wall (CW) and/or intracellular targets contribute to AP-induced lethality. We hypothesized that...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2020
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkaa1242